Background: The pathogenesis of premature biological ageing and chronic stress is increasingly linked not only to genetic predispositions but to modifiable external social and lifestyle factors. This study investigates the correlation between specific lifestyle variables (sleep, diet, physical activity), social determinants (isolation, socioeconomic status, work environment), and their collective impact on psychological status, perceived stress, and markers of premature ageing.

Methods: A cross-sectional observational study was conducted among 500 urban adults aged 30-55. Lifestyle and social factors were assessed using validated self-report questionnaires, alongside the Perceived Stress Scale (PSS) and the General Health Questionnaire (GHQ-12) for psychological status. Biological ageing was estimated using non-invasive phenotypic markers (e.g., skin elasticity scores) and self-reported health metrics. Data were analyzed using multivariate logistic regression models.

Results: High social isolation was strongly correlated with elevated PSS scores (p< 0.05) and poorer psychological status on the GHQ-12 (P<0.05). Individuals in the lowest quartile for sleep quality exhibited a 3.2-fold increase in phenotypic markers of premature ageing compared to those in the highest quartile. Work-related stress emerged as a significant mediator between socioeconomic status and accelerated ageing markers.

Conclusion: Adverse social environments and poor lifestyle choices significantly contribute to chronic psychological stress, acting as catalysts in the pathogenesis of premature ageing. Interventions targeting social connectivity and sleep hygiene are critical for mitigating these effects.